Isolation, Screening, Characterization And Application Of Biosurfactant By Achromobacter Xylos Strain GSR21 Producing Bacteria From Hydrocarbons Contaminated Soil

: Biosurfactants plays a key role in tertiary recovery (EOR), and production, excessive minerals discharge from water during petroleum refinery process, environmental utilization eco-friendly. Biosurfactant producing bacteria found to be sufficient hydrocarbon-polluted soil samples; it is expected more amounts of agrichemicals contemporary the clay. These establish itself region specific. So, this study we made an attempt identify characterize biosurfactant produced by achromobacter xylos strain GSR21 hydrocarbon polluted Andhrapradesh, India. A battery screening methods engaged were haemolytic activity, oil spreading technique, lipase emulsification index (E24), assay, tilting glass slide, blood haemolysis test, drop-collapsed foaming activity. The organism isolate was studied based on molecular, phenotypic, biochemical methods. Thin-layer chromatography I(TLC), Fourier transforms infrared spectroscopy (FTIR) gas chromatography-mass spectrometry (GC–MS) investigates used produced. isolated applied chosen hydrocarbons measure its emulsifying capacity.The phylogeny 16S rRNA classified as chromobacter GSR21. sequence secured has been accumulated GenBank covered accession number JQ746488. result obtained acknowledge high action with maximum (E24) 62 % compared 72% sodium lauryl sulfate (SLS). Moreover, appear activity against following hydrocarbons: diesel, methylbenzene, kerosene, dimethylbenzene, petrol. optimum cultural conditions (incubation time, carbon, pH, hydrocarbon, inoculum concentration, nitrogen, temperature) for growth analysed. characterized glycolipid using thin layer (TLC), while technique analyzed dodecanoic acid-undecyl ester.Present exhibited magnitude Achromobacter produce effectiveness different hydrocarbons. established held class, (TLC) analyses.